Influence of Temperature and Organics on ANAMMOX and Study on Kinetics Characteristics

Influence of Temperature and Organics on ANAMMOX and Study on Kinetics Characteristics

Liang Qiyu Jia Ruotan  Qu Wanxin  Zhang Deyuan  Ma Lin  Zhang Qun  Li Xianjin  Xie Yuanhua  Ma Yongguang  Zhu Tong 

School of Mechanical Engineering and Automation, Northeastern University, Shenyang 110819, Liaoning, China

Machinery Factory, Northeastern University, Shenyang 110819, Liaoning, China

School of Sciences, Northeastern University, Shenyang 110819, Liaoning, China

School of Metallurgy, Northeastern University, Shenyang 110819, Liaoning, China

Corresponding Author Email:
| |
| | Citation



The influence of temperature and organics on anaerobic ammonium oxidation (ANAMMOX) bacteria activity was investigated in an up-flow ANAMMOX reactor. The kinetics characteristics of cultured ANAMMOX bacteria were analyzed. The results showed that temperature had a significant effect on ANAMMOX bacteria activity, and it was very important to maintain a high temperature to obtain the high activity of ANAMMOX bacteria. There was substrata competition between the denitrification bacteria and ANAMMOX bacteria when organics in the influent. The half saturation constants for ammonium and nitrite of the ANAMMOX bacteria were 151.97 mg∙L-1 and 42.598 g∙L-1 respectively. The half inhibition constant for ammonium and nitrite of the ANAMMOX bacteria were 555.3 g∙L-1 and 200.6 g∙L-1 respectively.


ANAMMOX, Denitrification, Kinetics, Temperature, Organics

1. Introduction
2. Materials and Methods
3. Results and Discussion
4. Conclusions

[1] Qiao S., Yamamoto T., Misaka M., et al, “High-rate nitrogen removal from livestock manure digester liquor by combined partial nitritation–anammox process,” Biodegradation, vol. 21, no. 1, pp. 11–20, 2010. DOI: 10.1007/s10532-009-9277-8.

[2] Nozhevnikova A. N., Simankova M. V., Litti Y. V., “Application of the microbial process of anaerobic ammonium oxidation (ANAMMOX) in biotechnological wastewater treatment,” Applied Biochemistry & Microbiology, vol. 48, no. 8, pp. 667-684, 2012. DOI: 10.1134/S0003683812080042.

[3] Joss A., Salzgeber D., Eugster J., Litti Y. V., et al.,  “Full-scale nitrogen removal from digester liquid with partial nitritation and ANAMMOX in one SBR,” Environmental Science & Technology, vol. 43, no. 14, pp. 5301-6, 2009. 

[4] van der Star W. R., Abma W. R., Blommers D., et al., “Startup of reactors for anoxic ammonium oxidation: Experiences from the first full-scale anammox reactor in Rotterdam,” Water Research, vol. 41, no. 18, pp. 4149-4163, 2007. DOI: 10.1016/j.watres.2007.03.044 

[5] Strous M., Kuenen J. G., Jetten M., et al, “Key physiology of anaerobic ammonium oxidation,” Appl Environ Microbiol, vol. 65, no. 7, pp. 3248-3250, 1999. 

[6] State Environmental Protection Administration, Water and wastewater monitoring and analysis method, fourth, Beijing, China: Environmental Press, 2002, pp. 258-282.

[7] Isaka K., Sumino T., Tsuneda S., “High nitrogen removal performance at moderately low temperature utilizing anaerobic ammonium oxidation reactions,” Journal of Bioscience & Bioengineering, vol. 103, no. 5, pp. 486-490, 2007. DOI: 10.1263/jbb.103.486.

[8] Capdeville B., Cornier J. C., Deguin A. A. D., et al., “Study of factors controlling nitrite build-up in biological processes for water nitrification,” Water Science & Technology, vol. 26, no. 5-6, pp. 1017-1025, 1992. 

[9] Volokita M., Belkin S., Abeliovich A., et al, “Biological denitrification of drinking water using newspaper,” Water Research vol. 30, no. 4, pp. 965-971, 1996. DOI: 10.1016/0043-1354(95)00242-1.

[10] Liu Jinling, Zhong Yuming, Xie Zhiru, et al, “Metabolism of anaerobic ammonium oxidizing (anammox) bacteria with organic carbon addition,” Acta Scientiae Circumstantiae, vol. 29, no. 10, pp. 2041-2047, 2009.

[11] Yang Yang, Zuo Jianwu, Shen Ping, et al, “Influence of temperature pH value and organic substance on ANAMMOX sludge,” Environmental Science, vol. 27, no. 04, pp. 691-695, 2006. 

[12] Gee C. S., Suidan M. T., Pfeffer J. T., “Modeling of nitrification under substrate-inhibiting conditions,” Journal of Environmental Engineering, vol. 116, no. 1, pp. 18-31, 1990.