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Amperometric biosensors for determining cyanide which are accurate, cheap and practical have been made. The biosensor used a modified bentonite as a electerode membrane. Current measured by a potensiostat with a Cyclic Voltametry method. Study of calcination temperature on pillarization of bentonite and thickness of bentonite membrane was conducted to get electrode with the best performance. The performance was tested using : Cathecol linearity, maximum inhibition percentage, cyanide linearity, detection limit and sensitivity. The data showed that electrode with membrane of bentonite pillarized at 250 C and the thickness is 5 mg/mL had the best performance.
Biosensor, Cyanide, Pillared-Bentonite, Tyrosinase
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