OPEN ACCESS
This paper reports on an electrochemical biosensor to detect the hybridization specificity by using host-guest recognition technique. A hairpin DNA with a dabcyl molecule which is typical guest molecule to b-Cyclodextrins (b-CD) at the 3’-terminus and a NH2 linked at the 5’-terminus as the probe DNA. The probe DNA was immobilized on the PdS nanoparticle to construct a double-labeled probe (DLP) and could selectively hybridize with its target DNA. A b-CD modified Poly(N-acetylaniline) glassy carbon electrode was used for capturing dabcyl in DLP. Without binding with target DNA, the DLP keep stem-loop structure and block dabcyl enter into the cavity of b-CD on electrode. However, a target-binding DLP is incorporated into double stranded DNA, causing loop-stem structure opened and dabcy l could be easy captured by b-CD which brought DLP on electrode surface. With electrochemical measurement, the signal come from Pd2+ be used for target DNA quantitative analysis.
DNA, electrochemical, cyclodextrins, double-labeled probe, host-guest recognition
We gratefully acknowledge the financial support from JiangXi University of Traditional Chinese Medicine of China.
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